Figure 4.

MiR-125b enhances macrophage function. A) BMMs expressing the vectors MG or MG-125b were co-cultured with ovalbumin-specific OT1 T cells with or without ovalbumin for 72 hours. The percent CD8⁺CD25⁺ T cells are shown in the left panel. Concentration of IL-2 (pg/ml) produced by the T cells in the supernatant is shown in the right panel. Data represents the mean with SEM of 3 biological replicates per group. B) The percent AnnexinV⁺ EL4-Fluc cells after 94 hours of co-culture with control or miR-125 over-expressing macrophages in the presence of media alone or lipopolysaccharide (LPS). A representative flow cytometric plot of the LPS-treated group is shown. Data is expressed in mean with SEM of 1–3 experimental samples per group. C–E) EL4-Fluc cells were subcutaneously co-injected with LPS-activated control or miR-125b over-expressing macrophages into albino C57Bl/6 mice. Tumor surface area in cm² was monitored from day 9–12 (C). The relative intensity of luminescence (D) and weight (E) of the EL4 tumors were measured on day 12. Data represents the mean plotted with SEM of eight mice per group. Representative of two independent experiments.