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. Author manuscript; available in PMC: 2012 Nov 15.
Published in final edited form as: J Immunol. 2011 Oct 3;187(10):5255–5267. doi: 10.4049/jimmunol.1101186

FIGURE 1.

FIGURE 1

COX-2, but not COX-1, is upregulated in the ileum following peritonitis-induced polymicrobial sepsis. RNA was isolated from ileums of COX-2+/+ mice following sham or CLP (n=2-3), reverse-transcribed, and qPCR performed for COX-2 (A) and COX-1 (B). Results were normalized to endogenous control 18S (p<0.05 by the Mann-Whitney test). (C) Protein was harvested from ileums of COX-2+/+ mice (n=2-4) following sham or CLP and Western blot performed for COX-2 and COX-1. (D) Bar graphs represent protein levels (normalized for α-tubulin) as determined by densitometry. Protein levels are expressed as fold induction compared with COX-2+/+ mice following sham (p<0.05 by the Kruskal-Wallis one-way ANOVA test followed by Dunn’s post test). (E) Representative COX-2 immunostaining of ileums harvested from COX-2+/+ and COX-2−/− mice 24 h following sham or CLP. Arrows represent positively stained inflammatory cells in the lamina propria and arrowheads represent positively stained epithelial cells in the ileum.