Table 1.
Mascot Search Results for HLE B-3 Cells (Control vs. UVB Exposed)
| Spot | Protein | Accession No. (Gi) | Assigned Spectra (n) | MW (kDa) | Fold-Change |
||
|---|---|---|---|---|---|---|---|
| C1 vs. C2 | C1 vs. UVB | C2 vs. UVB | |||||
| 2348 | Heat shock protein 90 kDa-α | 154146191 | 9 | 85 | −1.24 | −2.2 | −1.8 |
| Heat shock 90 kDa protein 1-β | 20149594 | 7 | 83 | ||||
| 80 K-H protein | 182855 | 2 | 59 | ||||
| 2562 | Caldesmon 1 isoform 5 | 15149465 | 22 | 61 | 1.06 | 2.17 | 2.02 |
| Inner membrane protein mitochondrial isoform 1 | 154354964 | 15 | 84 | ||||
| Ezrin | 46249758 | 5 | 69 | ||||
| Procollagen-lysine 2-oxoglutarate 5-dioxygenase 3 | 4504517 | 4 | 85 | ||||
| ZYX protein | 33870614 | 3 | 62 | ||||
| 2614 | LaminA/C isoform | 27436946 | 41 | 74 | −1.2 | −1.82 | −1.53 |
| DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3 | 62087546 | 16 | 73 | ||||
| Glycosyltransferase 25 domain cont. 1 | 31377697 | 8 | 72 | ||||
| 2771 | Myristoylated alanine-rich protein kinase C substrate | 153070260 | 7 | 32 | −1.27 | −2.78 | −2.22 |
| Protein phosphatase 1G (6) | 405999 | 6 | 59 | ||||
| 3880 | β-Actin | 14250401 | 13 | 41 | 1.01 | 2.25 | 2.19 |
| HNRPF protein | 16876910 | 12 | 46 | ||||
| Eukaryotic translation initiation factor 4A isoform 1 | 4503529 | 9 | 46 | ||||
| Nuclear distribution gene C homolog | 5729953 | 3 | 38 | ||||
| Vimentin | 62414289 | 3 | 54 | ||||
| TXNDC5 protein | 30354488 | 3 | 40 | ||||
| 5375 | Triosephosphate isomerase | 136066 | 7 | 27 | −1.2 | 1.78 | 2.1 |
| Hsp27 1 | 4504517 | 5 | 23 | ||||
| Endoplasmic reticulum protein 29 isoform 1 precursor | 5803013 | 3 | 29 | ||||
| 6-Phosphogluconolactonase | 6912586 | 2 | 28 | ||||
| 5378 | Hsp27 | 4504517 | 2 | 23 | −1.08 | 2.36 | 2.53 |
| 5380 | Hsp27 | 4504517 | 17 | 23 | −1.23 | −1.75 | −1.44 |
| Peroxiredoxin 6 | 4758638 | 13 | 25 | ||||
| Triosephosphate isomerase | 136066 | 7 | 27 | ||||
| 5389 | Peroxiredoxin 6 | 4758638 | 11 | 25 | −1.39 | −2.38 | −1.73 |
| CLE | 55613379 | 9 | 28 | ||||
| Triosephosphate isomerase | 136066 | 7 | 27 | ||||
| Hsp27 | 4504517 | 5 | 23 | ||||
| Phosphoglycerate mutase | 387016 | 3 | 29 | ||||
| Endoplasmic reticulum protein 29 isoform 1 | 5803013 | 2 | 29 | ||||
| 5920 | Myosin regulatory light chain MRCL2 | 15809016 | 5 | 20 | −2.57 | −4.73 | −1.86 |
| 7094 | S100 calcium-binding protein A4 | 4506765 | 9 | 12 | 1.14 | 5.35 | 4.62 |
Two controls (C1 and C2) and one UVB sample were compared on the same gel. All the entries had 100% identification probability except for 6-phosphogluconolactonase, which had a 98% identification probability, and TXDNC5, which had 99% protein identification probability.