Table 5.
Mascot Search Results for Human Lenses from a 62-Year-Old Donor Exposed to UVB Radiation in the Presence and Absence of UV-Blocking Contact Lenses
| Spot | Protein | Accession No. | Assigned Spectra (n) | MW (kDa) | Fold-Change |
|
|---|---|---|---|---|---|---|
| C vs. UVB | C vs. UVB + UV-Blocking Contact Lens | |||||
| 4612 | Sorbitol dehydrogenase | 1583520 | 4 | 38 | −7.49 | −2.94 |
| αB-crystallin | 4503057 | 3 | 20 | |||
| Glyceraldehyde 3 phosphate dehydrogenase | 31645 | 3 | 36 | |||
| βS-crystallin | 258660 | 2 | 21 | |||
| 4614 | Glyceraldehyde 3 phosphate dehydrogenase | 31645 | 4 | 36 | −6.91 | −2.93 |
Single-gel comparison of protein spots in control and UVB-exposed human lenses from a 62-year-old donor. Note that this analysis was consistent with several proteins that changed in abundance upon exposure to UVB in the young human lenses: αB-crystallin, glyceraldehyde 3 phosphate dehydrogenase, and βS-crystallin. Identification probability was 100% for all proteins except βS-crystallin, which was 99%.