Figure 1.
Western blotting of vesicular monoamine transporter 2 (VMAT2) in the striatum of autopsied human brain. (A) Representative immunoblots of the tissue standard comprised of pooled human putamen and caudate samples probed with the rabbit polyclonal antibody raised against a C-terminus 19-amino-acid peptide of human VMAT2 (AB1767 from Chemicon) and the mouse monoclonal antibody (clone 9E11, subtype IgG1) raised against recombinant, full-length human VMAT2. The four VMAT2 bands detected by AB1767 in the striatum were assigned as bands I, IIa, III, and IV, respectively. Note the absence of bands III and IV by 9E11. (B) Curve fitting of the four standard curves. The mean ratios of levels of VMAT2 bands IIa, III, and IV versus I in the tissue standard from five determinations were 0.529±0.033, 0.358±0.023, and 0.115±0.009, respectively.