Figure 11.

Significant decrease in the accumulation of autofluorescent material in the RPE of an RPE65 postmortem donor eye. Human cryosections of both a matched control (A, C) and an affected RPE65 donor (B, D–G) were observed on epifluorescence in the green channel (FITC filter, excitation 495 nm/emission 519 nm). RPE from the RPE65 mutant retina displayed significantly decreased autofluorescent granules in the macula (B) and periphery in the inferior (D), superior (E), temporal (F), and nasal (G) quadrants when compared with the control RPE (A). Scale bar, 200 μm.