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Effect of temperature on hydrolysis of chitosan employing α-amylase initially (a) and then glucoamylase (b)
(a) Initial reaction conditions with α-amylase: 4 h, pH 5.0, 80 U/g of α-amylase; (b) Subsequent reaction conditions with glucoamylase: 8 h, pH 4.5, 4000 U/g of glucoamylase. Data are shown as mean±SD (n=9)
