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. 2011 Nov 4;5:15. doi: 10.3389/fncir.2011.00015

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Copyright © 2011 Bertrand and Cazalets.

This is an open-access article subject to a non-exclusive license between the authors and Frontiers Media SA, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and other Frontiers conditions are complied with.

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Detection of cholinergic commissural interneurons in spinal cord slices. Photomicrographs of commissural interneurons (CINs) retrogradely labeled with Texas red (TxR), applied ventrally to the central canal and immunopositive for CholineAcetylTransferase (ChAT) in lamina VII (A) and close to the central canal (B). The areas boxed in (A,B) are shown at a higher magnification in (a,b) photomicrographs. Arrows point to the double-labeled CINs. (C) A normalized spinal cord transverse section illustrating the location of all the double-labeled CINs observed. The area boxed is shown at a higher magnification in the inset. [Calibration bars: (A–C), 200 μm; (a,b), 50 μm; inset, 100 μm].