Table 3.
Analysis of Retinal Protein Expression in Human Glaucoma: Selected Protein Kinases
| Symbol | Protein Name | RefSeq | Fold Change |
|---|---|---|---|
| IKKE | Inhibitor of kappa-B kinase epsilon | NP_054721 | 1.9* |
| IKKG (NEMO) | NF-κB essential modulator, inhibitor of kappa-B kinase gamma | NP_003630 | 3.4* |
| RIPK1 | Receptor (TNFRSF)-interacting serine/threonine kinase 1 | NP_003795 | 2.2* |
| RIPK2 (CARD3) | Receptor-interacting serine/threonine kinase 2 | NP_003812 | 1.1 |
| ROCK1 | Rho-associated protein kinase 1 | NP_005397 | 2.3* |
| RAF1 (c-Raf) | Raf proto-oncogene serine/threonine-protein kinase 1 | NP_002871 | 1.4 |
| STK17A | Serine/threonine kinase 17a (apoptosis-inducing) | NP_004751 | 1.9* |
| STK25 | Serine/threonine kinase 25 (oxidant stress response kinase 1) | NP_006365 | 1.6 |
| MAP4K1 | Mitogen-activated protein kinase kinase kinase kinase 1 isoform 2 | NP_009112 | 1.2 |
| MAP4K2 (GCK) | Mitogen-activated protein kinase kinase kinase kinase 2 | NP_004570 | 6.4* |
| MAP4K4 | Mitogen-activated protein kinase kinase kinase kinase 4 | NP_004825 | 1.1 |
| MAP3K3 (MEKK3) | Mitogen-activated protein kinase kinase kinase 3 | NP_002392 | 1.2 |
| MAP3K4 (MEKK4) | Mitogen-activated protein kinase kinase kinase 4 | NP_005913 | 1.1 |
| MAP3K5 (MEKK5, ASK1) | Mitogen-activated protein kinase kinase kinase 5 | NP_005914 | 2.2* |
| MAP3K6 (MAKK6, ASK2) | Mitogen-activated protein kinase kinase kinase 6 | NP_004663 | 1.3 |
| MAP3K7 (MEKK7) | Mitogen-activated protein kinase kinase kinase 7 | NP_663304 | 1.7 |
| MAP3K14 (NIK) | Mitogen-activated protein kinase kinase kinase 14 | NP_003945 | 2.3* |
| MAP3K18 (TAOK3) | Mitogen-activated protein kinase kinase kinase 18 | NP_057365 | 2.4* |
| MAP2K2 (MKK2, MEK2) | Mitogen-activated protein kinase kinase 2 | NP_109587 | 1.5 |
| MAP2K6 (MKK6, MEK6) | Mitogen-activated protein kinase kinase 6 | NP_002749 | 2.8* |
| MAP2K7 (MKK7) | Mitogen-activated protein kinase kinase 7 | NP_660186 | 1.4 |
| MAPK1 (ERK2) | Mitogen-activated protein kinase 1 (variant 1) | NP_002736 | 4.4* |
| MAPK1 (ERK2) | Mitogen-activated protein kinase 1 (variant 2) | NP_620407 | 1.3 |
| MAPK3 (ERK1) | Mitogen-activated protein kinase 3 | NP_002737 | 3.0* |
| MAPK7 | Mitogen-activated protein kinase 7 | NP_002740 | 1.5 |
| MAPK8 (JNK1) | Mitogen-activated protein kinase 8 | NP_620634 | 2.9* |
| JIP2 | Mitogen-activated protein kinase 8 interacting protein 2 | NP_036456 | 1.1 |
| MAPK10 (JNK3) | Mitogen-activated protein kinase 10 isoform 3 | NP_620446 | 1.1 |
| MAPK13 (p38d) | Mitogen-activated protein kinase 13 | NP_002745 | 1.3 |
| MAPKAPK5 | Mitogen-activated protein kinase-activated protein kinase 5 | NP_003659 | 1.2 |
| PAK2 | P21-activated kinase 2 | NP_002568 | 2.6* |
| PAK 5/7 | P21-activated kinase 5/7 | NP_065074 | 1.8 |
| JAK2 | Tyrosine-protein kinase Janus kinase 2 | NP_004963 | 1.5 |
| JAK3 | Tyrosine-protein kinase Janus kinase 3 | NP_000206 | 1.7 |
Retinal protein samples obtained from human donor eyes with (n = 10) or without (n = 10) glaucoma were individually analyzed by quantitative LC-MS/MS. All listed proteins were identified with high confidence (greater than 99.0% probability assigned by the Protein Prophet algorithm) based on at least two identified peptides.
*
Significant difference in protein expression between glaucomatous and control samples (Mann-Whitney rank sum test; P < 0.05).