Table 4.
Analysis of Retinal Protein Expression in Human Glaucoma: Proteins Linked to Apoptosis
| Symbol | Protein Name | RefSeq | Fold Change |
|---|---|---|---|
| BCL(X)L | Bcl2-like 1 | NP_612815 | 1.9* |
| BAX | Bcl2-associated × protein | NP_620116 | 4.3* |
| BCL2L2 | Bcl2-like 2 | NP_004041 | 1.6 |
| BCL2L10 | Bcl2-like 10 | NP_065129 | 1.4 |
| BIM | Bcl2-like 11 | NP_619527 | † |
| BCL2L13 | Bcl2-like 13 | NP_056182 | 1.1 |
| BID | BH3 interacting domain-containing death agonist | NP_932070 | † |
| ASPP1 | Apoptosis-stimulating protein of p53, 1 | NP_056131 | 1.2 |
| TP53BP1 | P53-binding protein 1 | NP_005648 | 1.3 |
| CYTC | Cytochrome c, somatic | NP_061820 | 1.2 |
| AIFM1 | Apoptosis-inducing factor 1 | NP_665811 | 2.9* |
| AIFM2 | Apoptosis-inducing factor 2 | NP_116186 | 1.6 |
| ENDOG | Endonuclease G | NP_004426 | 3.0* |
| APAF1 | Apoptotic peptidase activating factor 1 | NP_863651 | 1.3 |
| CASP8 | Caspase 8 | NP_001219 | 1.7 |
| CASP9 | Caspase 9 | NP_001220 | 2.1* |
| CASP10 | Caspase 10 | NP_001221 | 1.4 |
| BCL10 | Caspase recruitment domain-containing apoptotic signaling protein | NP_003912 | 2.1* |
| CARD7 (NALP1) | Caspase recruitment domain-containing family member 7 | NP_127497 | 1.1 |
| CARD8 | Caspase recruitment domain-containing family member 8 | NP_055774 | 1.6 |
| CARD9 | Caspase recruitment domain-containing protein member 9 | NP_434700 | 1.4 |
| CARD10 (BIMP1) | Caspase recruitment domain-containing family member 10 | NP_055365 | 1.7 |
| CARD11 | Caspase recruitment domain-containing family member 11 | NP_115791 | 1.1 |
| CARD12 (IPAF) (NLRC4) | Caspase recruitment domain-containing protein member 12 | NP_067032 | 1.2 |
| PDCD10 | Programmed cell death 10; apoptosis-related protein 15 | NP_009148 | 2.7* |
| THAP1 | THAP domain-containing nuclear proapoptotic factor | NP_060575 | 2.0* |
| PAR4 | Prostate apoptosis response protein 4 | NP_002574 | 2.2* |
| ACIN1 | Apoptotic chromatin condensation inducer 1 | NP_055792 | 1.3 |
| DFFA (ICAD) | DNA fragmentation factor alpha | NP_004392 | 2.7* |
| CAPNS1 | Calpain small subunit 1 | NP_001740 | 3.8* |
| CAPN2 | Calpain 2 catalytic subunit | NP_001739 | 1.7 |
| CAPN6 | Calpain 6 | NP_055104 | 1.8* |
| CAPN9 | Calpain 9 | NP_006606 | 1.5 |
| CAPN10 | Calpain 10 | NP_075571 | 2.0* |
| CAST | Calpastatin isoform c, calpain inhibitor | NP_775084 | 2.3* |
| GRP78 (BIP) | 78 kDa glucose-regulated protein, heat shock 70kDa protein 5 | NP_005338 | 5.4* |
| DNAJC10 | DnaJ (Hsp40) homolog C 10 | NP_061854 | 2.2* |
| ERO1LB | Endoplasmic reticulum oxidoreductin 1-like beta | NP_063944 | 2.8* |
| PDI | Protein disulfide isomerase A1 | NP_000909 | 1.4 |
| PDIA6 | Protein disulfide isomerase A6 (ERP5) | NP_005733 | 3.6* |
| ERP29 | Endoplasmic reticulum protein 29 | NP_006808 | 3.3* |
| ERN1 | Serine/threonine-protein kinase/endoribonuclease IRE1 | NP_001424 | 2.5* |
| ATF6 | Activating transcription factor 6 | NP_031374 | 2.7* |
Retinal protein samples obtained from human donor eyes with (n = 10) or without (n = 10) glaucoma were individually analyzed by quantitative LC-MS/MS. All listed proteins were identified with high confidence (greater than 99.0% probability assigned by the Protein Prophet algorithm) based on at least two identified peptides.
*
Significant difference in protein expression between glaucomatous and control samples (Mann-Whitney rank sum test; P < 0.05).
†
Proteins detectable only in glaucomatous samples.