Figure 2.

Man does not deplete ATP in wild-type Arabidopsis seeds. Seeds were plated out at d −2 in the absence (control, ♦) or in the presence of 0.5 (▪), 7.5 (▴), or 50 (×) mm Man. After being submitted to a 48-h cold treatment at 4°C to promote germination, they were placed at 22°C at d 0. Measurements were taken at 24-h intervals to determine the ATP and protein levels in the seedlings. Approximately 50 seeds or seedlings were used for each measurement per experiment. prot., Protein.