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Clodronate-mediated depletion of macrophages reduces OVA/RV induced cytokine expression. Lung mRNA was extracted and corresponding cDNA subjected to quantitative real time PCR. (N=4 mice per group, bars represent mean±SEM, *different from sham group, †different from OVA/RV/ PBS liposome group, P<0.05, one-way ANOVA.)
