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. Author manuscript; available in PMC: 2011 Nov 4.
Published in final edited form as: J Immunol. 2010 Jul 19;185(4):2525–2535. doi: 10.4049/jimmunol.1000286

Figure 8.

Figure 8

Macrophages from OVA-sensitized and -challenged mice show increased cytokine mRNA expression after RV stimulation ex vivo. BAL fluid was extracted from PBS-treated and OVA-sensitized and -challenged mice and seeded in 12-well plates. Cells were allowed to adhere to plates for 90 minutes. Adherent cells were subsequently infected with RV1B, or sham or media (controls). A. Eotaxin-1 expression was observed in adherent BAL cells from OVA-treated but not PBS-treated mice. Eotaxin-1 significantly increased following RV stimulation. B. IL-13. C. TNF-α. D. IL-4. E. IL-10. F. IFN-γ. (N=3-4, bars represent mean±SEM. Because some treatment conditions yielded no detectable mRNA expression, data were normalized to the condition with the lowest detectable mRNA signal. *different from respective sham group, †different from respective UV RV group, §different from respective PBS group, P<0.05, one-way ANOVA.)