Table 3.
Boechera miRNA nucleotides substituted with those of Arabidopsis/Oryza.
| Pre-miRNA | NSs | NN | ΔG | |
|---|---|---|---|---|
| Plant sp./Boechera | Natural HP | "Corrected" HP | ||
| Sexual Species | ||||
| bsex-MIR156a* | U/A | 75 | -21.6 | -19.3 |
| bsex-MIR157a | U/A | 75 | -21.6 | -19.3 |
| bsex-MIR396 | U/G | 143 | -55.9 | -56.8 |
| bsex-MIR400* | A/G | 76 | -12.0 | -11.7 |
| bsex-MIR403* | G/C | 75 | -18.7 | -15.5 |
| bsex-MIR414* (1) | U/A, A/G | 170 | -49.8 | -48.2 |
| bsex-MIR414 (2) | U/A | 221 | -52.2 | -56.4 |
| bsex-MIR414 (3) | U/A | 233 | -57.9 | -60.0 |
| bsex-MIR852 | U/G | 89 | -34.1 | -34.7 |
| Apomictic Species | ||||
| bapo-MIR156a*(1) | U/A | 66 | -19.8 | -15.6 |
| bapo-MIR156a*(2) | U/A | 105 | -27.9 | -26.7 |
| bapo-MIR157m* | A/G | 119 | -38.2 | -31.3 |
| bapo-MIR394a | C/A | 126 | -23.8 | -26.2 |
| bapo-MIR414* (1) | U/A, A/U | 208 | -35.2 | -34.2 |
| bapo-MIR414* (2) | U/A, A/G | 170 | -49.8 | -48.2 |
| bapo-MIR414* (3) | U/G | 104 | -30.5 | -29.2 |
| bapo-MIR415* | A/G, C/A | 135 | -33.8 | -30.3 |
| bapo-MIR861* | A/G, G/U | 233 | -79.4 | -71.4 |
HP, Hairpin; NN, Number of nucleotides hairpin length; NSs, Nucleotide substitutions. Asterisk indicates cases where "correction" of Boechera miRNA NSs led to less stem-loop stability due to decrease in ΔG. Where there are two or more pre-miRNAs with the same miRNA, they are distinguished by numbers in brackets.