Table 1.
Ingenuity Pathways Analysis of differentially expressed EKLF target genes
| Gene expression pattern | Ingenuity biologic pathway 1 | Ingenuity biologic pathway 2 |
|---|---|---|
| Progenitor-specific EKLF target genes | ||
| Pro > Ery | ||
| Hi > Lo = 190 | Carbohydrate metabolism, small-molecule biochemistry (score = 25; focus genes = 18) | DNA replication, cell cycle, cellular development (score = 21; focus genes = 16) |
| Lo > Hi = 26 | Molecular transport, nucleic acid metabolism (score = 17; focus genes = 8) | NA |
| Erythroblast-specific EKLF target genes | ||
| Pro > Ery | ||
| Hi > Lo = 692 | DNA replication, cell cycle (score = 37; focus genes = 31) | DNA replication, cellular assembly and organization (score = 35; focus genes = 30) |
| Lo > Hi = 246 | Cell maintenance, small-molecule biochemistry (score = 19; focus genes = 15) | Hematologic system development (score = 17; focus genes = 14) |
| Common EKLF target genes | ||
| Pro > Ery | ||
| Hi > Lo = 70 | Cell death, cell cycle (score = 32; focus genes = 17) | Cell development, cell-cell signaling (score = 18; focus genes = 11) |
| Lo > Hi = 27 | Gene expression, cell cycle (score = 13; focus genes = 7) | NA |
Pathway analysis of changes in the mRNA levels of EKLF target genes expressed in both progenitor cells (Pro) and erythroblasts (Ery). Those genes that are occupied by EKLF only in progenitor cells or erythroblasts are referred to as progenitor or erythroblast specific EKLF target genes. Genes that are occupied by EKLF in both progenitor cells and erythroblasts are referred to as common EKLF target genes. mRNA levels can change from high levels in Pro to lower levels in ERY (Hi > Lo) or vice versa. The number of genes with that particular expression pattern is indicated. The top one or two pathways identified by the Ingenuity Pathways Analysis program and the numbers of EKLF target genes in that pathway are indicated. A score of > 3 is considered significant.