Figure 1. Detection of LLOp:I-A^b-specific CD4⁺ T cells.

(A) Gate used to identify CD3⁺ non-T cell lineage⁻ T cells (left) and CD4⁺ and CD8⁺ T cells within that population (right) from the bound fraction after enrichment with LLOp:I-A^b+ tetramer. (B) CD4⁺ T cells (left) or CD8⁺ T cells (right) identified as in (A) from an uninfected B6 mouse with gates on LLOp:I-A^b+ cells. (C) CD4⁺ T cells from B6 mice at the indicated times after intravenous infection with Lm-2W bacteria with gates on LLOp:I-A^b+ cells. The percentages of cells in the indicated gates are shown. (D) Mean number (± SEM, n ≥ 3 for each data point) of CD4⁺ LLO:I-A^b+ T cells in the spleen and lymph nodes over the first 8 (left) or 440 days (right) after intravenous infection with Lm-2W bacteria.