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. Author manuscript; available in PMC: 2012 Oct 28.
Published in final edited form as: Immunity. 2011 Oct 20;35(4):583–595. doi: 10.1016/j.immuni.2011.09.009

Figure 6. Development of TCM cells depends on Bcl6.

Figure 6

(A) Plot of Bcl6 versus CXCR5 expression by wild-type (black dots) or Bcl6−/− (red dots) LLOp:I-Ab-specific cells from a wild-type plus Bcl6−/− mixed radiation bone marrow chimera 3 days after intravenous infection with Lm-2W bacteria with a scatter plot showing the percentage of Bcl6+ naïve CD4+ T cells from uninfected B6 mice (diamonds) or CXCR5− (filled circles) or CXCR5+ (open circles) LLOp:I-Ab-specific cells from wild-type mice 3 days after infection. (B) Number of wild-type (filled circles) or Bcl6−/− (open circles) CXCR5− or CXCR5+ LLOp:I-Ab-specific cells from 3 wild-type plus Bcl6−/− mixed radiation bone marrow chimeras, 3 days after intravenous infection with Lm-2W bacteria. *, p < 0.05. (C) Plots of CXCR5 versus PD-1 staining used to identify wild-type or Bcl6−/− LLOp:I-Ab-specific Th1 effector cells, T −/− CM precursor cells, and TFH cells from wild-type plus Bcl6 mixed radiation bone marrow chimeras 7 days after Lm-2W infection. T-bet expression by wild-type or Bcl6−/− LLOp:I-Ab-specific cells in these chimeras is also shown. (D) Number of LLOp:I-Ab-specific Th1, TCM, and TFH cells identified as in (C) from individual wild-type plus Bcl6−/− (left) or wild-type plus Bcl6+/- (right) mixed radiation bone marrow chimeras, 7 days after intravenous infection with Lm-2W bacteria. *, p < 0.05, ***, p < 0.001.