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. Author manuscript; available in PMC: 2011 Nov 4.
Published in final edited form as: Mol Cancer Ther. 2010 Aug 17;9(10):2785–2792. doi: 10.1158/1535-7163.MCT-10-0481

Figure 1.

Figure 1

Characterization of HCC827 isogenic cell lines. A, HCC827 isogenic cell lines (HCC827, HCC827-Mock, HCC827-HGF1 and -HGF2, and HCC827 GR5) were cultured overnight in medium containing 10% serum and then incubated for 24 h in serum-free medium, after which the culture supernatants were collected and assayed for HGF with an ELISA. Data are means ± SD from three independent experiments. B, HCC827 isogenic cell lines were cultured in medium containing 10% serum for 72 h in the presence of various concentrations of gefitinib, after which cell viability was assessed as described in Materials and Methods. The number of viable cells is expressed as a percentage of the value for untreated cells. Data are means ± SD from three independent experiments. C, HCC827 isogenic cell lines were incubated for 1 h with or without gefitinib (100 nM) in medium containing 10% serum, after which the cells were lysed and subjected to immunoblot analysis with antibodies to phosphorylated (p-) or total forms of EGFR, MET, AKT, or ERK or with those to β-actin (loading control).