Figure 4.

The lack of effect of 22-OH cholesterol (22OH Chol) on progesterone secretion in SR-BI KD cells. HGL5 cells were transfected with scrambled or SR-BI siRNA for 72 h, and then incubated with DMSO, Fo (10 μm), Fo + 22-OH cholesterol (20 μm), or 22-OH cholesterol for an additional 24 h. Media levels of progesterone were measured by RIA. The data represent the mean ± se of three independent experiments, with each experiment performed using duplicate wells. *, P < 0.01 compared with scrambled cells.