Table 2.
Direct cytotoxicity of freshly isolated cells from spleens and livers of liver graft recipients (B10 → C3H), tested against syngeneic (C3H), donor (B10) and third-party (BALB/c) targetsa
| Percent specific 51Cr release (mean cpm ± 1 SD) |
||||||||
|---|---|---|---|---|---|---|---|---|
| Time postTx | Animal | Liver graft-derived cells (E:T ratio) |
Spleen-derived cells (E:T ratio) |
|||||
| C3H |
B10 |
BALB/c |
C3H |
B10 |
BALB/c |
|||
| 100:1 | 100:1 | 30:1 | 100:1 | 100:1 | 100:1 | 100:1 | ||
| 1-2 Weeks | a | 10±3 | 37±3.3 | 16±2.5 | 10±3.2 | 12±2.3 | 1±0 | 15±1.5 |
| b | 2±1.1 | 56±5.4 | 26±4.9 | 10±2.1 | 1±0 | 1±0 | 1±0 | |
| 4 Weeks | a | NDb | 36±2.9 | 20±2.6 | ND | 1±0 | 2±1 | 5±1.8 |
| b | 1±0 | 30±3 | 18±2.9 | 1±0 | 7±2.7 | 3±1.5 | 1±0 | |
| 8-12 Weeks | a | 9 ± 3 | 35±3.9 | 12±4.2 | 8±2.7 | 12±2.6 | 14±1.9 | 4±2 |
| b | 1±0 | 20±3.3 | 10±3.6 | 3±1.3 | 1±0 | 1±0 | 3±1.2 | |
a
51Cr-labeled Con A blasts were used as targets.
b
ND, not done.