Table 1. Known Ookinete-Interacting Proteins Identified in DRM Fractions^a.

accession no.	annotation	evidence for role of molecule in Plasmodium development	reference
AGAP004809	GPI-anchored Aminopeptidase N	Anti-AgAPN1antibodies inhibited both P. falciparum (70–80%) and P. berghei (70–80%) oocyst development	(11)
AGAP003790	Annexin-like	Anti-ANXB9 antibodies inhibited of P. berghei (30–38%) oocyst development	(12)
AGAP003721	Annexin-like	Anti-ANXB10B antibodies inhibited P. berghei (36–40%) oocyst development	(12)
AGAP003722b	Annexin-like	Anti-ANXB10C antibodies inhibited 28.2–43.7% of P. berghei development in the midgut	(12)
AGAP006209	Carboxypeptidase B	Antibodies against CpbAg1 inhibited both P. falciparum and P. berghei development	(13)
AGAP010133	Scavenger Receptor, Croquemort Homologue	Knock-down of SCRBQ2 results in a 62.5% inhibition of P. berghei oocyst formation	(14)

^aAn. albimanus calreticulin (AaCrt) has been shown to localize to the apical surface of An. albimamus midguts and to interact with a recombinant form of the abundant P. vivax ookinete surface protein, Pvs25.(15) The An. gambiae homologue of AaCrt, AGAP004212, was present in DRMs but was not included in Table 1, since to date, there is no direct evidence by either RNAi knock-down or the use of anti-AaCrt antibodies demonstrating the involvement of Anopheles midgut surface expressed calreticulin in Plasmodium invasion and establishment in the mosquito.

^bOnly known ookinete-interacting protein not detected in the DRM proteome.