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. 2010 May 25;151(8):3985–3995. doi: 10.1210/en.2010-0029

Fig. 6.

Fig. 6.

Effect of lack of leptin-dependent STAT3 signaling on the expression of proinflammatory genes in the prepubertal mammary gland. A, MFPs were collected from female mice lacking leptin-dependent STAT3 signaling (s/s) or their WT counterparts at 4 and 6 wk of age. Total RNA was analyzed by real-time PCR for the mRNA abundance of MCP1, EMR1, TNFα, and IL6. Each bar represents the mean ± se of six to eight mice. The significant effect of the genotype × age (G×A) is given. Pairwise comparisons performed within each age are significant at P < 0.05 (*) or P < 0.001 (**). B, Proliferation of Scp2, Eph4, and HC11 mouse mammary epithelial cells was measured using thymidine incorporation. Cells were incubated under basal conditions in the absence or presence of IGF-I (100 ng/ml), TNFα (100 ng/ml), and IL6 (100 ng/ml). The significant effects are indicated. In the case of an interaction, pairwise comparisons performed in the absence or presence of IGF-I are significant at P < 0.05 (*). Each bar represents the mean ± se of three wells.