Figure 1.

SLHMG cells express neuropeptide receptors. Cells were lysed in reducing sample buffer, boiled, and separated on a 10% tris/glycine gel, followed by transfer to nitrocellulose. Immunoblots for four neuropeptide receptors, each representative of 3 to 5 individual blots, are shown. (A) Representative immunoblots for high-affinity VIP receptors, VPAC1 and VPAC2. A431 human skin epidermal cell line serves as positive control. (B) Representative immunoblots for mACh receptor proteins M2R and M3R. Human heart extract lysate (HHE) serves as positive control.