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. 1999 Mar;119(3):1033–1040. doi: 10.1104/pp.119.3.1033

Table II.

Responses of barley plants grown for 19 d under 12 h photoperiods to 10 min of EOD R, FR, R followed by FR (R/FR), or FR followed by R (FR/R) light

Treatment Floral Stage
Apex Length
No. of Tillers
Dry Wt
BMDR-1 BMDR-8 BMDR-1 BMDR-8 BMDR-1 BMDR-8 BMDR-1 BMDR-8
mm g
Control 3.9  ± 0.5a 2.0  ± 0.0b 12.9  ± 3.5a 1.8  ± 0.1b 1.3  ± 0.1a 1.3  ± 0.1a 0.175a 0.167a
R 3.8  ± 0.4a 1.9  ± 0.1b 13.6  ± 2.5a 1.5  ± 0.1b 0.9  ± 0.1a 1.0  ± 0.1a 0.134a 0.149a
FR 4.1  ± 0.4a 2.0  ± 0.0b 11.1  ± 2.1a 1.7  ± 0.1b 0.4  ± 0.1b 0.4  ± 0.1b 0.166a 0.134a
R/FR 4.0  ± 0.5a 2.0  ± 0.1b 8.1  ± 1.9a 1.6  ± 0.1b 0.2  ± 0.1b 0.4  ± 0.1b 0.146a 0.150a
FR/R 3.6  ± 0.5a 2.0  ± 0.1b 9.5  ± 2.5a 1.6  ± 0.1b 0.9  ± 0.1a 0.7  ± 0.1ab 0.166a 0.153a

Seeds were sown in plastic trays and subirrigated with full-strength Hoagland solution. All seeds were placed in a growth chamber in DD at 20°C ± 0.1°C for 4 d prior to transfer to a growth chamber set to 12 h of CW and 12 h of dark. At the end of each photoperiod plants were given a 10-min pulse of R, FR, R followed immediately by FR, or FR followed immediately by R. Following treatment for 18 d, the average fresh weight of the shoots was determined, and 10 plants that were within ±10% of the mean fresh weight were selected. These were then dissected under a dissecting microscope to determine the stage of floral development, the length of the inflorescence, and the number of tillers formed. Significance was established by analysis of variance. Different letters indicate a significant difference at P = 0.05.

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