Table 2.
Lengths of N-call stretches following mtDNA sequencing using MitoChip with GSEQ and sPROFILER.
| Sample Name | N-Call stretch lengths (bp) | Mutation found by conventional sequencing | |
|---|---|---|---|
| *GSEQ | *sPROFILER | ||
| mtDNA1 | 5, 6, 9, 13 | 4, 5 | 1 point mutation (in 5 base stretch) |
| mtDNA2 | 5, 6, 12 | 4, 5, 6 | 1 point mutation (in 6 base stretch) |
| mtDNA3 | 5, 6, 7, 11 | 4, 10 | 1 point mutation, 1 insertion (in 10 base stretch) |
| mtDNA4 | 5, 6, 11 | 4, 5 | 1 insertion (in 5 base stretch) |
| mtDNA5 | 5, 13 | 4, 5 | 1 insertion (in 5 base stretch) |
| mtDNA6 | 5, 7, 11 | 4, 5, 11 | 1 point mutation, 1 insertion (in 11 base stretch) |
| mtDNA7 | 5, 10 | 4 | Entire mtDNA sequenced: 1 point mutation (in 4 N-call stretch) |
| mtDNA8 | 5, 6, 8, 9, 10, 11, 14, 18, 20 | 4, 5, 6, 8, 10, 18, 20 |
1 point mutation (in 20 base stretch) 2 point mutations (in 18 base stretch) 1 point mutation (in 10 base stretch) |
| mtDNA9 | 5, 6, 9, 11, 12 | 4, 5, 8 | 2 insertions (in 8 base stretch) |
| mtDNA10 | 5, 7, 8, 9, 10 | 4, 5, 7, 9 | 1 point mutation (in 9 base stretch) |
| mtDNA11 | 5, 6, 8, 9, 12 | 4, 5, 8 | 1 point mutation (in 8 base stretch) |
| mtDNA12 | 5, 9, 10 | 4, 9 | 1 insertion (in 9 base stretch) |
| mtDNA13 | 5, 7, 8, 9, 10 | 4, 5, 9 | 1 point mutation (in 9 base stretch) |
| mtDNA14 | 5, 8, 10 | 4, 5 | No point mutation or indel (in 5 base stretch) |
| mtDNA15 | 5, 6, 7, 11 | 4, 5 | No point mutation or indel (in 5 base stretch) |
| mtDNA16 | 5, 7, 8, 10, 11 | 4, 6 | No point mutation or indel (in 6 base stretch) |
*Only stretches ≥ 4 bases identified in GSEQ are listed. For each sample only the longest N-call stretch was sequenced conventionally (except mtDNA7 and mtDNA8). Shorter N-call stretches occurred multiple times-as seen in Figure 1.