Table 2. Her2/neu expression and Her2/neu ECD release in the media supernatants of uterine serous tumours.
| RT–PCR |
Flow cytometry
|
sHer2/neu | ||||
|---|---|---|---|---|---|---|
| IHC | FISH | mRNA copy no. | % Gated | MFI | ng ml−1 per 96 h | |
| USPC-ARK-1 | 3+ | 2.5a | 2749.5 | 99.8 | 339.5 | 4.2 × 10−6 |
| USPC-ARK-2 | 3+ | 5.2a | 4478.9 | 99.9 | 710.3 | 4.9 × 10−6 |
| USPC-ARK-3 | 3+ | 4.7a | 4993.8 | 99.9 | 228.7 | 3.4 × 10−6 |
| USPC-ARK-9 | 3+ | 4.6b | 4326.5 | 99.3 | 285.7 | 9.1 × 10−6 |
| USPC-ARK-10 | 3+ | 4.3b | 549.8 | 98.8 | 25.4 | 1.6 × 10−6 |
| USPC-ARK-7 | 2+ | 1.3b | 45.6 | 94.9 | 47.4 | 0 |
| USPC-ARK-6 | 1+ | 0.9a | 44.3 | 93.4 | 13.0 | 0 |
| USPC-ARK-8 | 1+ | 1.6b | 17.6 | 83.2 | 24.8 | 0 |
| USPC-ARK-4 | 1+ | 1.6a | 52.8 | 95.2 | 10.8 | 0 |
| USPC-ARK-5 | 1+ | 1.4a | 95.3 | 83.8 | 18.7 | 0 |
| BT-474 | 3+ | Amplified | 897 | 99.9 | 654.9 | 6.4 × 10−6 |
| SK-BR-3 | 3+ | Amplified | 932 | 99.9 | 265.4 | 2.89 × 10−6 |
Abbreviations: FISH=fluorescent in situ hybridisation; IHC=immunohistochemistry; RT–PCR=real-time PCR; MFI=mean fluorescence intensity; USPC=uterine serous papillary adenocarcinoma; sHer2/neu=soluble Her2/neu.
a
FISH analysis performed on formalin-fixed, paraffin-embedded cell blocks obtained from primary cell lines in culture.
b
FISH performed on formalin-fixed, paraffin-embedded tissue blocks of the original tumour sample.