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. Author manuscript; available in PMC: 2011 Nov 4.
Published in final edited form as: J Immunol. 2007 Oct 1;179(7):4721–4731. doi: 10.4049/jimmunol.179.7.4721

FIGURE 2.

FIGURE 2

A primary infection of H. polygyrus (Hp) up-regulates Ym1, Arg1, and Fizz1 expression in macrophages (Mac) of multiple tissues. A–C, Macrophages (were purified from spleen, MLN, and colonic LP of helminth-infected mice at 3–4 wk after infection or from normal control mice. FACS analysis of the purified macrophages revealed that they were >95% F4/80+CD11b+ cells (data not show). D, Colonic tissues were collected from control and helminth-infected mice (7 days after H. polygyrus (H.p.) infection). Total RNA was isolated from the purified macrophages and colonic tissues. Ym1, Arg1, and Fizz1 expression was determined using realtime PCR. Values are the fold increase compared with baseline obtained from uninfected control mice. The data shown are from one of two experiments performed showing similar results.