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. 2011 Nov 4;6(11):e26644. doi: 10.1371/journal.pone.0026644

Figure 5. Interleukin-1 down regulates endogenous LRAT but not the ectopically expressed recombinant LRAT, indicating regulation is not at protein level.

Figure 5

Coding region of rat LRAT gene was cloned and confirmed by DNA sequencing. Recombinant rat LRAT was expressed by adenoviral vector (Ad.LRAT) in 293A cells, and GFP expressing adenoviral vector (Ad.GFP) was used as the control. The expression of LRAT protein was assessed by Western blot analysis. GAPDH was used as loading control. (B) HSCs were transduced by adenoviral vectors with MOI at 100. Three days after transduction, HSCs were treated with or without IL-1 (10 ng/ml) for additional 3 days. As shown by Western blot analysis, the endogenous LART but not recombinant one is down-regulated by IL-1, indicating the regulation is not at protein level. (C) Oil red O staining for each group of HSCs. As shown, ectopic expression of recombinant LRAT attenuated IL-1-indcued mobilization of lipid droplets in HSCs. Original magnification ×200.