Skip to main content
. 2011 Nov 4;6(11):e26644. doi: 10.1371/journal.pone.0026644

Figure 6. Identification of Kupffer cell secreted factor which suppresses LRAT expression in hepatic stellate cells as interleukin-1.

Figure 6

(A) A diagram of experimental design. (B) Conditioned medium was prepared by culture of primary rat Kupffer cells (KCs) for 3 days. A portion of the conditioned medium was added to HSC culture for 18 hours. The inhibitory effects of KC secreted factors on LRAT expression by HSCs were analyzed by Western blot. To demonstrate the contribution of IL-1 in the conditioned medium of KCs, IL-1 receptor antagonist (IL-1RA, at 1 µg/ml) was added to the KC-CM prior to the treatment for HSCs. GAPDH: loading control.