Figure 5. The Hnrpll mutation does not affect the up-regulation of CD122 in the developmental stages of NKT cell.

(A) Representative overlay histograms showing the expression of CD122 on the surface of NKT cells from the thymus of a wild type and Hnrpll ^thu/thu mouse. (B) Representative overlay histograms comparing CD122 expression on stage 1 (CD44^lo NK1.1^lo), stage 2 (CD44^hi NK1.1^lo) and stage 3 (CD44^hi NK1.1^hi) NKT cells in the thymus in wild type (left) and Hnrpll ^thu/thu mice (right). (C) Graph shows the geometric mean fluorescence intensity (MFI) of CD122 expression on stage 2 and 3 NKT cells and DP thymocytes in the thymus and NKT cells and TCRβ⁺ cells in the spleen. Bars represent the mean value of each group ± s.d. from one of two independent experiments with a group of n = 3–5 mice per group in each. (D) In vitro survival of NKT cells culture in the presence of varying concentrations of IL-15. Data shows the percentage of viable cells by 7-AAD exclusion after 3 days of culture for wild type (+/+) and Hnrpll ^thu/thu (thu/thu) thymic NKT cells. Graph from one of three independent experiments represents the average of viable cells recovered from duplicate cultures with 2 or 3 mice per group.