Table 1. Primer sequences used for mRNA quantification by real-time PCR.
Forward primer 5′–3′ | Reverse primer 5′–3′ | |
11β-HSD1 | GAA GAA GCA TGG AGG TCA AC | GCA ATC AGA GGT TGG GTC AT |
5αR1 | CTG TTT CCT GAC AGG CTT TGC | GCC TCC CCT GGG TAT CTT GT |
5βR | GCC TTT AAG CCT GGA GAG GAA | ACG TGG CAC ACA GAT TTG ATT |
GR | GGG TAC TCA AGC CCT GGA ATG | CCC GTA ATG ACA TCC TGA AGC T |
C/EBPα | GGC GGG AAC GCA ACA A | TCC ACG TTG CGC TGT TTG |
C/EBPβ | TCT ACT ACG AGC CCG ACT GC | AGG TAG GGG CTG AAG TCG AT |
GAPDH | CAA GTT CAA CGG CAC AGT CAA | TGG TGA AGA CGC CAG TAG ACT C |
11β-HSD1, 11β-hydroxysteroid dehydrogenase type 1; 5αR1, 5α-reductase type 1; 5βR, 5β-reductase; GR, glucocorticoid receptor; C/EBPα, CCAAT/enhancer-binding protein α; C/EBPβ, CCAAT/enhancer-binding protein β; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.