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. 2011 Nov 4;6(11):e25726. doi: 10.1371/journal.pone.0025726

Table 1. Primer sequences used for mRNA quantification by real-time PCR.

Forward primer 5′–3′ Reverse primer 5′–3′
11β-HSD1 GAA GAA GCA TGG AGG TCA AC GCA ATC AGA GGT TGG GTC AT
5αR1 CTG TTT CCT GAC AGG CTT TGC GCC TCC CCT GGG TAT CTT GT
5βR GCC TTT AAG CCT GGA GAG GAA ACG TGG CAC ACA GAT TTG ATT
GR GGG TAC TCA AGC CCT GGA ATG CCC GTA ATG ACA TCC TGA AGC T
C/EBPα GGC GGG AAC GCA ACA A TCC ACG TTG CGC TGT TTG
C/EBPβ TCT ACT ACG AGC CCG ACT GC AGG TAG GGG CTG AAG TCG AT
GAPDH CAA GTT CAA CGG CAC AGT CAA TGG TGA AGA CGC CAG TAG ACT C

11β-HSD1, 11β-hydroxysteroid dehydrogenase type 1; 5αR1, 5α-reductase type 1; 5βR, 5β-reductase; GR, glucocorticoid receptor; C/EBPα, CCAAT/enhancer-binding protein α; C/EBPβ, CCAAT/enhancer-binding protein β; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.