Figure 1. RBMY knockdown reduced the transformation and anti-apoptotic efficiencies of HepG2 cells.
(A) Quantitative RT-PCR analysis of RBMY in parental (G2), vector plasmid (VC), pSUPER-680, pSUPER-778, and pSUPER-914 plasmids transfected HepG2 cells. (B) Immuno-histochemical staining of HepG2 and the corresponding transfectants for RBMY protein. (C) Colony formation of parental or transfected HepG2 cells on soft agar. (D) Percentage of cell viability by MTT assay post 0.5 or 0.75 mM hydrogen peroxide (H2O2) treatment. Data were presented as mean±SD in four independent experiments. *p<0.05; **p<0.01.