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. 2011 Nov 4;6(11):e27164. doi: 10.1371/journal.pone.0027164

Figure 5. Micrographs of mutant strains of YBT-020 and their corresponding complementary strains.

Figure 5

(A) and (B) Strain BMBJA (genes orf1 and orf2 disrupted). (C) and (D) Complementary strain BMBJA/pBMB251B5 (carrying orf1 and orf2). (E) Complementary strain BMBJA/pBMB251B5A (carrying a frameshift at the AccI site in orf1). (F) Complementary strain BMBJA/pBMB251B5B (carrying a frameshift at the EcoRI site in orf2). (G), (H) and (I) strain BMBJB (genes orf3, orf4, and orf5 disrupted). (J) Complementary strain BMBJB/pBMB252B3 (containing orf3, orf4, and orf5). (K) Strain BMBJ1 (gene cry26Aa disrupted); (L) Complementary strain BMBJ1/pBMB0617 (carrying the gene cry26Aa). (A), (C) and (G) Electron micrographs of a thin sections during sporulation, arrowheads indicate exosporium. Scale bar = 0.5 µm. (B), (D), (E), (F), (K) and (L) after Growth for 48 h. (H) after growth for 36 h. (I) and (J) after growth for for 36 h, 100 h. Magnification, ×1,000.