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. 2011 Nov 4;6(11):e27164. doi: 10.1371/journal.pone.0027164

Table 3. Primers used for PCR.

Primer Sequence Construct(s) Use
cry26-F CCCTGGATCCGGAAATAAACGAACCTTCA pBMB0617A Heterologous expression
cry26-R CGCGAAGCTTTGGTGATGTTAAGCCCATAT
251B1 CCCGAGTACTTAAATAATAGCCT pBMB251B5 Heterologous expression
251B2 CCCAAAGCTTATAGGTACGATACTAC
251B4 CCAGAATTCATGCCCATCAACT pEMBJA Gene disruption
251B5 CGGTAGAATTCGAGTTGTACTTTCT
251B6 CAGGATCCGTATCGTACCTATA pEMBJB Gene disruption
251B7 CGCAAGCTTACCATCAAATGC
251B8 AAGGAATTCAACT GGGGT CCTTC pEMBJB Gene disruption
251B9 TACGAATTCGCGGCTCATAACT
cry26A AATGGATCCAAACGAACCTTCATTCA pEMBJ1 Gene disruption
cry26B GGCTGGATCCTTAACTTTGTATTTCC
CM1 AAAGAATTCTTCGCTACGCTCAAATC pEMBJ1 Gene disruption
CM2 TCAGAATTCTTCACCGTCATCACCGA