Table 3. Primers used for PCR.
Primer | Sequence | Construct(s) | Use |
cry26-F | CCCTGGATCCGGAAATAAACGAACCTTCA | pBMB0617A | Heterologous expression |
cry26-R | CGCGAAGCTTTGGTGATGTTAAGCCCATAT | ||
251B1 | CCCGAGTACTTAAATAATAGCCT | pBMB251B5 | Heterologous expression |
251B2 | CCCAAAGCTTATAGGTACGATACTAC | ||
251B4 | CCAGAATTCATGCCCATCAACT | pEMBJA | Gene disruption |
251B5 | CGGTAGAATTCGAGTTGTACTTTCT | ||
251B6 | CAGGATCCGTATCGTACCTATA | pEMBJB | Gene disruption |
251B7 | CGCAAGCTTACCATCAAATGC | ||
251B8 | AAGGAATTCAACT GGGGT CCTTC | pEMBJB | Gene disruption |
251B9 | TACGAATTCGCGGCTCATAACT | ||
cry26A | AATGGATCCAAACGAACCTTCATTCA | pEMBJ1 | Gene disruption |
cry26B | GGCTGGATCCTTAACTTTGTATTTCC | ||
CM1 | AAAGAATTCTTCGCTACGCTCAAATC | pEMBJ1 | Gene disruption |
CM2 | TCAGAATTCTTCACCGTCATCACCGA |