Figure 3.
CD34 expression on mucosal dendritic cells (DCs). (A) Flow cytometry histograms showing expression of CD34 on DCs (CD45+/CD11c+/MHCIIhi) cells isolated from either the bronchoalveolar lavage (BAL) (alveoli), lung, or mediastinal lymph nodes of mice that were exposed to Saccharopolyspora rectivirgula (SR) or vehicle (phosphate-buffered saline). These data are representative of four to five individual mice and of two independent experiments. (B) Relative CD34 mRNA expression found in SR-stimulated bone marrow–derived DCs (BMDDCs) and pulmonary DCs isolated from wild-type (wt) hypersensitivity pneumonitis (HP) mice. Results are expressed as fold of expression over naive cells.