Figure 5.

Development of hypersensitivity pneumonitis (HP) in bone marrow chimeric and W/W^v mice. (A) Total cells found in the bronchoalveolar lavage (BAL) of mice transplanted with wild-type (wt) or Cd34^−/− bone marrow and challenged with the Saccharopolyspora rectivirgula (SR) antigen. (B) Total number of CD11c⁺ cells found in the alveoli or parenchyma of bone marrow chimeric mice. (C) Percent of dendritic cells (DCs) expressing the maturation marker CD86 isolated from the parenchyma of bone marrow chimeric mice. (D) Lung vascular permeability in response to the SR antigen, as determined by total protein leakage in the alveoli and expressed as the BAL fluid/serum total protein ratio. (E) Total cells found in the BAL of mast cell–deficient W/W^v mice and their wt littermates challenged intranasally with the SR antigen. Data are presented as mean ± SEM and are representative of two separate experiments. n = 4–6 mice per group.