Figure 6.

Adoptive transfer of wild-type (wt) dendritic cells (DCs) into Cd34^−/− mice restores disease. (A) Representative example of CD34 expression in the two DC subtypes found in the splenic preparation (CD11b⁺ = myeloid; CD8⁺ = lymphoid). (B) Total inflammatory cells found in the bronchoalveolar lavage (BAL) of mice adoptively transferred with wt and Cd34^−/− DCs. Data are presented as mean ± SEM and are representative of four separate experiments. n = 4–6 mice per group. *P < 0.05, Cd34^−/− compared with wt. **P < 0.05, Cd34^−/− + wt DCs compared with Cd34^−/− alone. ***P < 0.05, Cd34^−/− + wt DCs compared with Cd34^−/− + Cd34^−/− DCs. (C) Flow cytometry plots representing fluorescein isothiocyanate (FITC)⁺ myeloid (CD11b⁺) and nonmyeloid (CD11b^neg)/CD11c⁺ lymph node cells. (D) Numbers of Cd11c⁺/FITC⁺ cells in the cervical lymph nodes isolated from wt and Cd34^−/− mice. (E) Splenic DCs were derived from wt and Cd34^−/− mice and examined for their ability to chemotax across matrigel-coated transwells in response to increasing concentrations of CCL19.