Figure 7.

Expression of human CD34 in Cd34^−/− mice restores disease sensitivity. (A) Isotype control and (B) CD34 expression in unstimulated and (C) 50 μg/ml Saccharopolyspora rectivirgula (SR)-stimulated human monocyte–derived dendritic cells (DCs). (D) Total number of bronchoalveolar lavage (BAL) cells, (E) percentage of leukocyte subsets, and (F) total number of lymphocytes, macrophages, and neutrophils/ml found in the BAL of wild-type (wt), Cd34^−/−, and Cd34^−/− mice expressing the human CD34 gene (Cd34^−/−/hCd34⁺). (G) Total number of DCs found in the alveoli and (I) parenchyma of wt, Cd34^−/−, and Cd34^−/−/hCd34⁺ mice. (H) Percentage of DCs in the parenchyma of wt, Cd34^−/−, and Cd34^−/−/hCd34⁺ mice. Data are presented as mean ± SEM, and are representative of three separate experiments. n = 6 mice per group. *P < 0.05.