Figure 1. Direct leptin to the rat VTA induced phosphorylation events.
Regulation of various Lepr pathways was evaluated 45 minutes after direct leptin infusion to the VTA of cannulated awake adult male rats. Ratios of phospho-/total signal were used to calculate the percent change from control tissue. Blots shown are representative. Open bars represent vehicle while black bars represent leptin treatment. A. pSTAT3 (Tyr-705) (n = 4) *, (P = 0.0077). B. pERK1/2 (Thr-202, Tyr-204) (n = 4) *, (P = 0.0252). C. pAKT (Ser-473) (n = 4). D. pAKT (Thr-308) (n = 4).