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. 2011 Nov 4;6(11):e27180. doi: 10.1371/journal.pone.0027180

Figure 2. U0126 abolished the firing response of dopamine neurons to leptin in mice.

Figure 2

See results section for statistical information. A. Representative traces of action potentials recorded before, during, and after the application of leptin to mouse VTA DA neurons in slices with (left panel) or without (right panel) U0126 (10 mM). Box, H-current recorded in VTA DA neurons. Hyperpolarizing voltage steps from −50 to −120 mV for 2 s generates a large/h current (>100 pA), the presence of which identifies dopamine neurons. B. A representative time course of the response of action potential frequency to leptin in the presence (open symbol) or absence (solid symbol) of U0126 in VTA DA neurons. The solid horizontal black line indicates the duration of leptin application. C. A summary of normalized frequency of action potentials before, during, and after the application of leptin in DA neurons in VTA slices pre-treated with U0126.