Figure 3. Bacterial growth and secretion of EPEC in the presence of CPAC.

Growth of EPEC and S. Typhimurium in the presence of 0 and 100 µg/mL CPAC was examined over 8 hours. CPAC had no effect on the growth of both bacterial strains (Figure 3a ). EPEC total proteins were resolved by 12% SDS-PAGE in the presence of increasing amounts of CPAC before probing for Tir protein on Western Blot (wells 1–4); 0, 25, 50, 100 µg/mL CPAC respectively). EPEC secreted proteins after 30 min incubation with 0, 25, 50, 100 µg/mL CPAC (supernatant, wells 5–8 respectively; pellet, wells 9–12 respectively). CPAC had no effect on the production of effector proteins, but readily precipitates proteins (Figure 3b ).