Figure 6. N-isobutyl decanamide confers protection against Botrytis cinerea attack.

Leaves from 20 day-old plants grown in soil were pre-incubated 24 h on solvent (control, white squares), or 30 µM N-isobutyl decanamide containing plates (black squares), transferred to decanamide-free plates and then inoculated with a 10 µl droplet of B. cinerea spores (5×10⁵ conidiospores/ml). The percentage of leaves with necrotic symptoms at 3, 4 and 5 days after inoculation was determined (A). Bars mean ± SE of 30 inoculated leaves from two independent experiments. Tissue damage caused by B. cinerea was measured at 5 days after inoculation (B). Data points represent average lesion size ± SE from 30 independent leaves, asterisks denote a significant difference from control leaves (P≤0.05) as determined by t test. Representative inoculated leaves at 5 days after inoculation were imaged (C, top panels) and trypan blue-stained, inoculation sites are shown (C, bottom panels).