Figure 1.

Fewer activated Inducible costimulator-deficient (ICOS^−/−) CD4 T cells are found in inflamed airways and lungs than wild-type cells. C57Bl/6 (B6) and ICOS^−/− mice were sensitized and challenged with inactivated Schistosoma mansonii eggs and soluble egg antigen (SEA). On Day 4 after challenge, mice were killed, and airway (bronchoalveolar lavage; BAL) and lung (tissue digest) cells were analyzed by flow cytometry. (A) CCR3⁺SSC^high eosinophils and CD3⁺CD4⁺ T cells from BAL. (B) CD4⁺ T cells and CD44⁺CD62L⁻ activated CD4⁺ T cells from the lungs. This experiment was repeated three times (n ≥ 4 for each group). *P < 0.05, **P < 0.01, and ***P < 0.001.