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. 2011 Mar 25;45(4):867–873. doi: 10.1165/rcmb.2010-0352OC

Figure 5.

Figure 5.

CO-induced LC3B is dependent on ROS production in A549 cells. (A) A549 cells were pretreated with 5 mM NAC for 30 minutes followed by CO treatment (250 ppm) for 24 hours. LC3B protein levels were determined by Western blotting. (B) A549 cells were treated with Mito-TEMPO (100 μM) for 24 hours in the presence of CO (250 ppm) or room air. (C) HBE cells were pretreated with 5 mM NAC for 30 minutes followed by CO treatment (250 ppm) for 24 hours, or were treated with Mito-TEMPO (100 μM) for 24 hours in the presence of 250 ppm CO or room air; β-actin was used as the standard (A–C). (D) HBE cells were treated with 5 mM NAC or Mito-TEMPO (100 μM) for 24 or 48 hours. Lactate dehydrogenase (LDH) levels were detected in cell culture supernatants by colorimetric assay (see Materials and Methods). Data are presented as average (±SE) of three independent experiments. *P < 0.05, #P < 0.01 (A–B and D).