Figure 4.

Effect of added soluble P-selectin or IL-5 on expression of epitopes for activation-sensitive anti-β₁ integrin mAb N29 or activation-sensitive anti-β₂ integrin mAb24. N29 (A, B) or mAb24 (C, D) epitope expression on eosinophils in whole blood after preincubation with (thick line) or without (normal line) added soluble P-selectin (0.1 μg/ml) (A, C) or IL-5 (50 ng/ml) (B, D). The mouse IgG₁ isotype is shown as a thin line. (E) N29 epitope expression on eosinophils in matched whole blood (closed symbols) and purified blood eosinophils (open symbols) from a normal subject after preincubation with different concentrations of added soluble P-selectin. Representative results of experiments with five donors (see text and Figure E4 in the online supplement).