Figure 5.

N29 epitope expression on blood eosinophils or eosinophilic leukemic EoL-3 cells in suspension before and after adhesion to vascular cell adhesion molecule (VCAM)-1. Representative of results with four donors (eosinophils) or three experiments (EoL-3 cells). N29 epitope expression was measured on purified blood eosinophils from an allergic asthmatic donor (A), EoL-3 cells (B), or eosinophils in whole blood from an allergic asthmatic donor to which soluble P-selectin (0.1 μg/ml) had been added (C). Measurements were made on the original cell population before adhesion to VCAM-1 (“total,” normal line) or on cells nonadherent to coated soluble 7-domain VCAM-1 (thick line). The mouse IgG₁ isotype is shown as a thin line. Coating concentrations of VCAM-1 were 10, 1, and 30 μg/ml in A, B, and C, respectively. The fact that all distributions in (C) as well as in Figures 4A–4D are to the right compared with those in A and B is due to different cytometer settings for whole blood to accommodate neutrophils and monocyte distributions compared with for purified cells or cell lines.