TABLE 2.
SUMMARY OF IMMUNOFLUORESCENT MICROSCOPIC STAINING OF EOSINOPHILS FOR ACTIVATED β1 INTEGRIN, P-SELECTIN, AND PLATELET MARKERS
| Antibody | Pattern | Colocalization with others | Figure |
| Anti-total β1 | Patchy and diffuse, in all eos | More extensive than 9EG7 | NS |
| Anti-active β1 (9EG7) | Patchy and diffuse, in many but not all eos | Less extensive than total β1 | NS |
| Partly overlapping with P-selectin, also P-selectin-negative areas | 3A,C | ||
| 3A,C | |||
| Anti-P-selectin | Punctate or patchy, in many but not all eos | Partly overlapping with 9EG7 | 3B,C |
| Partly overlapping with TSP-1, also TSP-1-negative areas | 3D,F | ||
| 3D,F | |||
| Anti-TSP-1 | Patchy, in some but not all eos | Partly overlapping with 9EG7, similar to αIIb | 3E,F |
| E2A | |||
| Anti-αIIb | Similar to TSP-1 | Similar to TSP-1 | E2D |
| Anti-eos MBP | Granules, in all eos | Not overlapping with the others | E2B |
Definition of abbreviations: eos, eosinophils; EPO, eosinophil peroxidase; MBP, major basic protein; NS, not shown; TSP, thrombospondin.
This table summarizes the data shown in Figures 3 and E2 (in the online supplement) as well as data not shown. Immunofluorescence microscopic staining of cytospun whole leukocytes from seven subjects with nonsevere allergic asthma was performed as described in Materials and Methods in the online supplement. Eosinophils were identified by morphology (see Figure E2C, E) and positive staining for eosinophil MBP (Figure E2B) or EPO (not shown).