Figure 5.

ErbB4 and Stat5a bind to the Sftpb promoter. (A) Primers were designed for the Sftpb promoter, encompassing approximately 200–base pair intervals. (B) Cells were mock-stimulated (C) or FCM-stimulated (F). A chromatin immunoprecipitation (ChIP) assay was performed, and DNA bound to ErbB4 or Stat5a was analyzed by semiquantitative PCR amplification. The chromatin was immunoprecipitated, using antibodies directed against ErbB4 and Stat5a. The input DNA was used as positive control (Input), and a negative control with no antibody (Mock) was included. ErbB4 binds to all three sites in the Sftpb promoter region. Stat5a requires stimulation by FCM to bind to all three sites.