Figure 2.

(A–C). Lung lavage cellularity and cytokine/chemokine release is acutely reduced in TLR2-deficient mice after a one-time challenge with low- and high-concentration DE. Lung lavage fluid mean concentration of total cells (A) and cell differential (B) 5 hours after single intranasal inhalation exposure of DE (0% [saline], 2.5%, and 12.5%) in WT and TLR2 KO mice. (C) Cell-free mean levels of lung lavage fluid supernatant cytokines/chemokines collected 5 hours after exposure to DE. Error bars represent SE (n = 4–6 mice per group). ^#Statistically significant between respective DE-treated and saline-treated group. *P < 0.05 and **P < 0.01 indicate statistical significance between WT and KO DE-treated mice.