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. 2011 Nov 4;6(11):e27355. doi: 10.1371/journal.pone.0027355

Figure 7. Epigenetic regulation of P16-INK4A tumor suppressor gene.

Figure 7

a) Expression profile of P16-INK4A in treated and untreated cell lines; in two cancerous cell lines (MDA-MB231 and SKBR3) no expression was detected. b) Expression profile of P16-INK4A within all analyzed passages according to the normalized Ct values (ΔCt). c) Methylation proportion of informative CpG sites of P16-INK4A in SKBR3 cell line. d) Full gene sequencing of P16-INK4A revealed a heterozygote transition mutation in 5′UTR and a homozygote transversion mutation in 3′UTR of the gene. e) Pathway analysis predicts the role of has-miR-24 (MIRN24) as a down-regulator of the P16-INK4A. f) Expression profile of has-miR-24 in the non-aggressive breast cancerous cell line (SKBR3) versus control breast cell line (HB2). g) Comparison of miR-24 recognition site with the location of found mutations in the mature P16-INK4A mRNA.